Estimation of Protein content in some selected plants using the Lowry’s method
Ami Makavana1*, Mohini Patel2, Ranna Chaudhary3 and Ruby Patel4
1-4Department of Botany, M.N. College, Visnagar - 384315 India *CorrespondingAuthor Email: amimakvana31@gmail.com
ABSTRACT
This study aims to conduct a comprehensive phytochemical analysis of protein content and its concentrations derived from selected plant species through the utilization of acetone extraction, adhering strictly to the Lowry’s method. The acetone extract from various parts of the selected plants on the college campus was meticulouslyprepared through a systematic process involving thorough cleaning, precise chopping, careful drying, and ultimately grinding into a fine powdered form. The presence of protein content was diligently assessed, and the concentration of unknown proteins in the samples was accurately determined, employing a standard curve derived from BSA as a benchmark, utilizing the Lowry’s method. Acomprehensive selection of 12 plants, representing 4 distinct families, was meticulously gathered, revealing that the protein concentration was maximally recorded in Albizia lebbeck L. Benth. at 0.85 mg per ml, while the minimum was observed in Cucurbita maxima Duchesne at 0.11 mg per ml. Furthermore, the other selected plants exhibited notable levels of protein content. The selected plants possess the potential to serve as a significant source of protein, with applications in various pharmaceutical industries and as components of vegan protein products.