Molecular characterization of potent antimicrobial metabolite producing actinomycetes from the soil of Kalaburagi region
Shweta Mallikarjun and *M Vidyasagar Gunagambhire
Department of PG Studies and Research in Botany, Gulbarga University, Kalaburagi 585106 (India) shwetapatil409@gmail.com & *gmvidyasagar@gmail.com Mob no: 9449258812 *Corresponding author: Dr. M Vidyasagar Gunagambhire Department of Botany, Gulbarga University, Kalaburagi 585106 (India) Email: gmvidyasagar@gmail.com
ABSTRACT
This study focuses on isolating and characterizing actinomycetes from soil samples collected in the Kalaburagi region and assessing their antimicrobial activity against Candida and various bacterial spp. The isolated actinomycetes were performed by antagonistic activity against Candida. The potent isolate VSG-15 ethyl acetate crude extract displayed a significant zone of inhibition against C. albicans MTCC1966, Candida haemulonii MTCC8303, and Candida tropicals MTCC1406 as compared to the Standard drug. The crude extract also showed significant antibacterial activity was recorded against Brevibacillus brevis and Enterococcus faecalis Based on morphological, biochemical characteristics and 16S rRNA sequencing the isolated strain was identified as Streptomyces levis (VSG-15). The present study reveals that Streptomyces levis are potential organisms for the production of anticandidal and antibacterial agents against the clinical test organism.