Actinomycetes as potent Antibacterial agents and Biofilm inhibitors
Shivani Patil1 and Tessy Anu Thomas*
Department of Microbiology, Krupanidhi Degree College Bangalore - 560035 1,*Email: patil.shivani1001@gmail.com Address of Corresponding author Dr. Tessy Anu Thomas* Department of Microbiology Krupanidhi Degree College, Bangalore. 560035 Email: tesaby08@gmail.com* Orcid id: 0000-0002-1388-4525
ABSTRACT
Oral health influences the general quality of life and poor oral health is linked to chronic conditions and systemic diseases which continues to be a major health problem worldwide. Dental caries and periodontitis are the two most common oral diseases and are closely related with the oral microbial flora that includes bacteria such as Streptococcus mutans and other anaerobic bacteria like Porphyromonas and Bacteroides spp which are also potent biofilm producers. On the verge of increased incidence of antibiotic resistance, the aim of the study was to evaluate the potency of actinomycetes as potent antibacterial agents and biofilm inhibitors. In context to this, actinomycetes strains were isolated from soil samples from various parts of India and identified in the generic level by following the Dichotomous key followed by assessing the antimicrobial potency against Streptococcus mutans and Streptococcus oralis by Kirby Bauer method. The strains were also assessed for their biofilm inhibiting potency by Crystal violet method. Among all the strains tested, the actinomycetes isolated from Gujarat exhibited potent antimicrobial and antibiofilm activity which subsequently resulted to be identified as Streptomyces roseofulvus through 16S rRNA using Sanger sequencing with 98.96% and by PCR amplification.