Optimization of Cultural conditions for Protease production by Bacillus subtilis using Banana peel waste
R. Sneka1 , Marius K. Somda2 , P. Jacquline Rosy3 , J. Sivasubramaniyan4 and P. Saranraj1
1PG and Research Department of Microbiology, Sacred Heart College (Autonomous), Tirupattur – 635 608 (India) 2Laboratory of Biochemistry, Biotechnology, Food Technology and Nutrition (LABIOTAN), Department of Biochemistry and Microbiology, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso, (West Africa) 3Department of Chemistry, IFET College of Engineering (Autonomous), Villupuram - 605108 (India) 4Department of Physics, The Madura College, Madurai - 625011 (India) Corresponding Author E.mail: microsaranraj@gmail.com
ABSTRACT
Enzymes are biocatalysts for most chemical reactions that occur inside living cells and are necessary for life. In the present research, the Bacillus subtilis in Tomato rhizosphere soil samples was isolated by Spread plate method and identified by Microscopic examination, Plating on Culture medium and Biochemical tests. Efficient Bacillus subtilis isolates was screened for its Proteolytic activity on Skimmed milk agar plates and the Bacillus subtilis isolate with the Highest clear zone was selected for the further studies.