Isolation, Identification and Characterization of Bacillus sp., from Soil Sample
*R. Manju and **S. Guruvayurappan
Department of Microbiology, Hindusthan College of Arts & Science, Coimbatore - 641028 (India)
ABSTRACT
Bacillus is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum Bacillota, with 266 named species9 . The term is also used to describe the shape (rod) of other so-shaped bacteria; and the plural Bacilli is the name of the class of bacteria to which this genus belongs. Bacillus species can be either obligate aerobes which are dependent on oxygen, or facultative anaerobes which can survive in the absence of oxygen. Cultured Bacillus species test positive for the enzyme catalase3 if oxygen has been used or is present. The organisms in soil sample were serially diluted and inoculated onto the agar plates. The isolated organisms were identified by means of various techniques. Gram staining technique was performed and small Gram positive bacilli were observed. Endospore staining technique was performed and red colour bacilli along with green colour spores were observed. Various Biochemical tests were done for the Identification of bacteria1 . Further identification of bacterial strain was done by Sanger sequencing method. The microbial strain was identified as Bacillus subtilis.