Analytical and Formulative Approaches to Doshaghna Churna: Development of a Modified Patch and Validation by HPTLC and FTIR
Taruna Rathod1 , Sangeeta H. Toshikhane2* , Kavita Ishrani1 and Jay Patel1
1,2*Department of Panchakarma, Parul Institute of Ayurved, Parul University, Limda, Vadodara - 391760 (India)
ABSTRACT
Doshaghna Lepa is classically mentioned as Shothahara Lepa (Punarnava, Devdaru, Sunthi, Sigru & Sarshapa) in Sarngadhara SaChita, by Acharya Sarangadhara. The present study aims to develop modified Doshaghna herbal patch and scientifically evaluate the phytochemical constituents of Doshaghna Churna and Doshaghna extract using advanced instrumental techniques such as Fouriertransform infrared (FTIR) spectroscopy and High-Performance Thin Layer Chromatography (HPTLC). FTIR analysis confirmed the presence of multiple bioactive functional groups including hydroxyl, carbonyl, and aromatic compounds, indicating the presence of flavonoids, tannins, alkaloids, and glycosides. HPTLC analysis at 254 nm and 366 nm revealed reproducible chemical fingerprints with significant peaks and consistent Rf values, validating batch-to-batch uniformity and phytochemical richness