Assessment of cytogenotoxic potential of Ethion in Zebrafish (Danio rerio F. Hamilton, 1822) by using micronucleus assay
Vinayan. K. V. and *R.V. Tijare
Institute of Science, Civil lines, R.T Road, Nagpur - 440001 (India) *Corresponding author: rvtijare@gmail.com
ABSTRACT
Ethion used in agriculture commonly reaches the non-target organisms like aquatic life and raises environmental concerns which has cautioned the use and many countries have put it under restricted use. Genotoxic impact of Ethion on the non-target aquatic life was assessed by exposing. Zebrafish (Danio rerio) to three sublethal doses, i.e. 0.340 mg/L, 0.476 mg/L and 0.612 mg/L at four-time intervals, viz., 24 hours, 48 hours, 72 hours and 96 hours. The study established that Ethion exerts a genotoxic effect on the erythrocytes of Zebrafish, causing the appearance of micronuclei in the erythrocytes after exposure in a manner that was both concentration-dependent and time-dependent. At higher concentrations the changes were frequent, and the exposure time had a direct correlation to the abnormal behaviour which is statistically significant.