In-Vitro Analysis of Bioactive Compounds from Senna auriculata (L.) Roxb. (Traditional Herb) for Wound Healing Applications
1*D S Srimathi and 2R Manju
1*,2Department of Microbiology, Hindustan College of Arts and Science, Coimbatore - 641028 (India) *Corresponding author Email: 1 srimathinov1st@gmail.com
ABSTRACT
Medicinal plants are widely recognized as natural alternatives for wound healing due to lower toxicity, safety and biocompatibility. This current study examines the comparative biological activity of Senna auriculata leaves, flowers and roots for wound healing properties. The ethanolic extract of Senna auriculata plant parts were examined for phytochemical analysis, chromatographic profiling and assessed for antimicrobial, antioxidant, and anti-inflammatory activities. Qualitative phytochemical analysis confirmed the presence of tannins, alkaloids, saponins, flavonoids, phenols, glycosides, proteins, and root-specific quinone. The TLC study represented six different Rf values with bioactive compounds, and column chromatography purified seven different fractions of extracts. HPTLC analysis revealed major bioactive compounds, flavonoids and phenols. Quantitative phytochemical analysis revealed the highest levels of phenols and flavonoids in the root extract, followed by the flower extract. The flower and root extracts demonstrated antibacterial effects even at low concentrations. In DPPH and protein denaturation assays, the root extracts exhibited stronger activity compared to the flower extract. Overall findings support that root and flower extract contains the specific bioactive compounds, flavonoids and phenols, responsible for stronger biological activities in managing skin infections and emphasize its potential as a natural agent for wound healing properties.