Mechanistic evaluation of plant-mediated silver nanoparticles on MDR Bacillus cereus through comparative proteomics
Sasi Lekha S1* and S. Antony2
1*,2Department of Microbiology, Malankara Catholic College, Mariagiri, Kaliyakavilai - 629153 (India) Affiliated to Manonmaniam Sundaranar University, Tirunelveli, India *Corresponding author : Sasi Lekha .S, E mail: reshmirp24@gmail.com
ABSTRACT
The proteomic response of multidrug-resistant Bacillus cereus to silver nanoparticles (AgNPs) made using extract from Boerhavia diffusa is assessed in this work. The presence of bioactive metabolites involved in the production of nanoparticles was verified by LC-MS analysis. Ten proteins were found to be significantly changed (p < 0.05) by proteomic profiling using 2D-GE and MALDI-TOF MS. The proteins most commonly downregulated were those involved in membrane transport (SecA, GerN), nucleotide biosynthesis (PurL), DNA repair (AddB, MutS2), and detoxification (FosB). Transmembrane transport, nucleic acid binding, and ATP binding were all disrupted, according to Gene Ontology analysis. The results show that AgNPs generated from B. diffusa have a multitarget antibacterial impact by inducing oxidative stress and suppressing vital cellular processes in MDR B. cereus.