Pharmaceutical and Phytochemical Standardization of Lakshadi Guggulu with Special Emphasis on HPTLC Fingerprint Profiling and Its Therapeutic Relevance in Osteoarthritis
1Mansi Deore and Vaishali Deshpande2
1,2Department of Kayachikitsa, Parul Institute of Ayurveda and Research, Parul University, Vadodara - 391760 (India)
ABSTRACT
Lakshadi Guggulu is one of the classical polyherbal Ayurvedic drug formulations that are commonly employed in treating disorders of bone metabolism, healing fractures, or degenerative joint diseases. Despite the common practice of using Lakshadi Guggulu in traditional medicine, there is little evidence of employing modern analytical techniques in its standardization. In the present study, the effort has been made to use High Performance Thin Layer Chromatography (HPTLC) in the standardization of the polyherbal formulation Lakshadi Guggulu and correlate the results with various bioactive compounds, which are responsible for the efficacy of the drug in treating certain inflammatory disorders like osteoarthritis. The methanolic formulation was analyzed at different concentrations using an ultraviolet detector at 254 nm as well as a fluorescent detector at 366 nm. The consistent chromatographic pattern indicates the uniform presence of resin acids, guggulsterones, phenolic compounds, withanolides, and lipid fractions in the formulation. The dominant appearance of medium-polarity compounds like resinous fractions, fluorescent steroidal lactones, supports evidence of the efficacy of the drug in treating inflammatory diseases like osteoarthritis.