Gas chromatography-Mass Spectromerty (GC-MS) Profiling and Standardization of Punarnavashtak Ghana prepared with Bhavna (Trituration) of Gomutra : Exploring its Therapeutic Potential in Metabolic Dysfunction -Associated Fatty Liver Disease
1*Devkanya Khatediya, Vaishali Deshpande2*
1,2Department of Kayachiktsa, Parul Institute of Ayurveda And Research, Parul university, At – Ishwarpura, Post– Limda , Tal. – Waghodia, Dist. – Vadodara - 391760 (India) 1Contact No. - 7974318848 2Contact No. – 9096082950 vaishali.deshpande86209@paruluniversity.ac.in *Corresponding author: Dr. Devkanya Khatediya Email – khatediyadevkanya@gmail.com
ABSTRACT
Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) is a prevalent disorder characterized by hepatic steatosis with metabolic dysregulation such as obesity ,insulin resistance, and metabolic syndrome. Ayurvedic formulation with hepatoprotective potential require scientific validation for broader acceptance. Punarnavashtak Ghana, a classical polyherbal formulation, is traditionally indicated in Yakrit Vikar (liver disorder). Bhavna(trituration) with Gomutra is described in Ayurveda to enhance bioavailability and therapeutic potency. To standardize Gomutra processed Punarnavashtak Ghana and characterize its volatile and semi-volatile phytoconstituets using Gas chromatography-Mass Spectrometry (GC-MS) to explore its therapeutic relevance in MAFLD. Punarnavashtak Ghana was prepared according to classical Ayurvedic procedures and subjected to three Gomutra Bhavana cycles. GC–MS analysis was performed in Total Ion Chromatogram mode with spectral matching using the NIST23 library