Evaluation of Therapeutic Potential of Haridradi Ghanavati in Management of Hypothyroidism Through High-Performance Thin Layer Chromatography (HPTLC) Analysis
1Palakkumari Paramar and 2Naresh Kore
1,2Department of Kayachikitsa, Parul Institute of Ayurveda and Research, Parul University, Vadodara - 391670 (India)
ABSTRACT
Hypothyroidism is characterized by insufficient thyroid hormone synthesis and is linked to inflammation, weight gain, dyslipidemia, and slowed metabolism. Haridradi Ghanavati (HGV) is a traditional polyherbal compound detailed in Ashtanga Hrudaya, which may have therapeutic value for hypothyroidism. A proven analytical method for creating phytochemical fingerprints and guaranteeing formulation authenticity, purity, and quality is High-Performance ThinLayer Chromatography (HPTLC). Haridradi Ghanavati were made using classical Ayurvedic method after raw materials were authenticated. Physicochemical parameters including ash values, loss on drying, tablet hardness, disintegration, and dissolution were assessed. Sample was prepared by weighing 5gm of sample in a beaker and Methanolic extracts was added into it, HPTLC analysis at concentrations of 5–20 µL using silica gel 60 F254 plates. Chromatograms were visualized under 254 nm and 366 nm to identify characteristic phytochemical markers. Curcumin, termerone, zingiberene, berberune, liquiritigenin, isoliquiritigenin, glycurrhizin, tannins,phenolic glycosides, coumarins and tridoids were identified as major phytochemicals. The compounds were known to decrease thyroidal inflammation, antioxidants, improve metabolic rate, control weight gain, hypothalamic-pituitary-thyroid axis regulation. The obtained fingerprint demonstrates efficient extraction and the presence of signature bioactive markers from each ingredient. The dominance of curcuminoids, berberine, and liquorice flavonoids highlights the therapeutic potential of the formulation, especially in inflammatory and metabolic conditions relevant to hypothyroidism.